Laboratory manual for acid-fast microscopy

Mycobacteriology Laboratory Manual v LIST OF ABBREVIATIONS AND ACRONYMS AFB Acid fast bacilli AST Antimycobacterial susceptibility testing; see DST BAP Blood agar plate BD Becton Dickinson BSC Biological Safety Cabinet BSL Biosafety level COA Certifi cate of analysis CDC US Centers for Disease Control and Prevention CFR US Code of Federal Regulations CFU Colony forming unitFile Size: 2MB.  · No headers. Basic Microbiology laboratory for upper division college students with color diagrams and very simple instructions. The manual covers lab safety, how to use a microscope, aseptic transfer of bacteria, simple, gram, capsule, acid fast, spore, and negative staining, environmental requirements of bacteria, antibiotic susceptibility testing, bacterial . For acid-fast bacilli (AFB) microscopy and HIV rapid tests, these individuals who have been trained “on the job” can perform as well as formally trained laboratory technicians, but the training programmes must be well thought out and supported at all levels, and need routine EQA to monitor performance. 18, 29 The formal training for.

Furthermore, as clinicians highly rely on the acid-fast smear results in order to choose the adequate therapeutic, isolation, and contact investigation steps, all clinicians should be aware of the method of acid-fast microscopy and proficiency testing used in their laboratory. The evaluator needs to have a good understanding of acid-fast bacilli (AFB) smear microscopy and good laboratory practice (GLP). Methods assessed include specimen collection, smear preparation, acid-fast stain procedure, reading, and reporting. The evaluator must understand appropriate methods and ensure they are being followed. The use of. Manual of Clinical Microbiology. Acid-fast smear evaluation and reportinga Report No. of AFB seen by staining method and magnification Fluorochrome stain Ziehl-Neelsen Stain X X X No AFB seen 0 0 0 Doubtful; repeat 1 – 2/30 F (1 sweep) 1 -2/70 F ( sweeps) 1 – 2/ Fb (3 sweeps)c 1+ 1 – 9/10 F 2 – 18/ 50 F 1 – 9/ F.

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